Skin from a factory - this has long been the dream of pharmacologists, chemists and doctors.

Research has an urgent need for large quantities of ‘skin models’, which can be used to determine if products such as creams and soaps, cleaning agents, medicines and adhesive bandages are compatible with skin, or if they instead will lead to irritation or allergic reactions for the consumer.

But artificial skin is rare. “The production is complex and involves a great deal of manual work. At this time, even the market's established international companies cannot produce more than 2,000 tiny skinpieces a month.

With annual requirements of more than 6.5 million units in the EU area alone, however, the industrial demand far exceeds all currently available production capacities,” reports Jörg Saxler. Together with Prof. Heike Mertsching, he is coordinating the “Automated Tissue Engineering on Demand” project within the Fraunhofer-Gesellschaft.

Tissue engineering is still in its infancy. “Until now, the offer was limited predominantly to single-layer skin models that consist of a single cell type,” explains Mertsching, who heads the Cell Systems Department at the Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB.

“Thanks to developments at our institute, the project team has access to a patent-protected skin model that consists of two layers with different cell types. This gives us an almost perfect copy of human skin, and one that provides more information than any system currently available on the market.”

An interdisciplinary team of Fraunhofer researchers is currently developing the first fully automatic production system for two-layer skin models. “Our engineers and biologists are the only ones who have succeeded in fully automating the entire process chain for manufacturing two-layer skin models,” explains Saxler, who is from the Fraunhofer Institute for Production Technology IPT where he is responsible for technology management and heads the “Life Science Engineering” business unit.

In a multi-stage process, first small pieces of skin are sterilized. Then they are cut into small pieces, modified with specific enzymes, and isolated into two cell fractions, which are then propagated separately on cell culture surfaces. The next step in the process combines the two cell types into a two-layer model, with collagen added to the cells that are to form the flexible lower layer, or dermis. This gives the tissue natural elasticity.

In a humid incubator kept at body temperature, it takes the cell fractions less than three weeks to grow together and form a finished skin model with a diameter of roughly one centimeter. The technique has already proven its use in practice, but until now it has been too expensive and complicated for mass production. Mertsching explains, “The production is associated with a great deal of manual work, and this reduces the method's efficiency.”

The project team, in which engineers, scientists and technicians from four Fraunhofer institutes are cooperating, is currently working at full speed to automate the work steps. The researchers at the IGB and the Fraunhofer Institute for Cell Therapy and Immunology IZI are handling the development of the biological fundamentals and validation of the machine and its sub-modules.

Experts from the Fraunhofer Institute for Manufacturing and Automation IPA and the Fraunhofer Institute for Production Technology IPT are taking care of prototype development, automation and integration of the machine into a complete functional system.

"Skin from a factory - this has long been the dream of pharmacologists, chemists and doctors. (Credit: Image courtesy of Fraunhofer-Gesellschaft)"

Source: Fraunhofer-Gesellschaft